This study was undertaken to evaluate the efficacy of a homeopathic complex in the management of true anoestrus in crossbred cows. Six anoestrus cows were treated with a homeopathic complex (Calcarea phosphorica 30c, Aletris farinosa 30c, Pulsatilla 30c, Aurum muriaticum natronatum 30c, Sepia 30c and Phosphorus 30c in equal proportion, 15 pills twice daily orally for 10 days). Six animals acted as control without any treatment.Treatment was 100% effective in inducing oestrus in anoestrus cows with mean interval of 27.5±5.3 days. All animals conceived and overall conception rate was 54.5% with 1.83 services per conception. In the homeopathic complex treated group, increased serum oestradiol concentration (20.88±5.60 to 27.80±7.28 pg/ml) was observed compared to the pretreatment (11.71±2.06 pg/ml) and control value (10.43±1.77 to 13.94±3.14 pg/ml). The homeopathic complex medicine may be effective and economical in the treatment of true anoestrus condition in cows.